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KMID : 0613820020120020121
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Journal of Life Science
2002 Volume.12 No. 2 p.121 ~ p.128
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The Effect of the Membrane Fluidity of Bellflower(Platycodon grandiflorum A.) Fractions on Liposomal Phospholipid Membranes
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Kang Bo-Young
Bae Song-Ja
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Abstract
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The object of this study was to investigate the effect of membrane fluidity of bellflower(Platycodon grandiflorum A. DC, ; PG) fractions in phosphatidylcholine(PC) liposomes, measured with high-sensitivity differential scanning calorimetry(DSC). We used dipalmitoylphosphatidylcholine(DPPC) bilayers which make most stable liposomes among the other phosphatidylcholine. The sample PG was extracted and fractionated to five different types ; butanol(PGMB), ethylacetate(PGMEA), ethylether(PGMEE), hexane (PGMH) and methanol(PGMM). Among five different solvent fractions, the PGMEE, PGMEA, PGMH and PGMM fractions markedly affected the thermotropic properties of DPPC liposomes, broadened and shifted the thermograms, and reduced the cooperative unit. It might be said that the incorporation of PGMEE, PGMEA and PGMH in DPPC liposomes were located in the hydrophobic core of DPPC bilayers and, PGMM and PGMB in the hydrophilic core of DPPC bilayers. These results suggest that certain substances in the PGMEE, PGMEA and PGMH fractions might have biologically significant effect on the membrane fluidity.
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KEYWORD
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DPPC, Platycodon grandiflorum A, DC, fluidity, liposome, DSC
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